4500 U of T4 RNA Ligase 2 (30,000 U/mL) and 10X Ligation Buffer (1 x 1.5 mL)
The T4 RNA Ligase 2 (4500 U) is intended for molecular biology applications. This product is neither intended for the diagnosis, prevention or treatment of a disease, nor has it been validated for such use either alone or in combination with other products.
Features
- Ligates nicks on double-stranded RNA
- Ligates 5ʹ phosphate and 3ʹ hydroxyl of RNA
Product Details
T4 RNA Ligase 2 catalyzes phosphodiester bond formation between a 5’ phosphate and 3’ hydroxyl termini of RNA. The preferred substrate is nicked double-stranded RNA, but other nicked nucleic acid hybrids can also be sealed. T4 RNA ligase 2 requires ATP for activity unless the substrate is pre-adenylated on the 5’ end (1-5).
Supplied in:
10 mM Tris-HCl, 100 mM NaCl, 0.1 mM DTT, 0.1 mM EDTA, 50% glycerol (pH 7.5 at 25°C)
Supplied with:
10X Ligation Buffer (B6030): 500 mM Tris-HCl, 100 mM MgCl2, 50 mM DTT, 10 mM ATP (pH 7.6 at 25°C)
Performance
- Storage temperature: –25°C to –15°C
- Molecular weight: 37.6 kDa
| Test | Amount tested | Specification |
|---|
| Purity | n/a | >99% |
| Specific activity | 500 U | >120,000 U/mg |
| Single-stranded exonuclease | 500 U | <5.0 % released |
| Double-stranded exonuclease | 500 U | <1.0 % released |
| Double-stranded endonuclease | 500 U | No conversion |
| E. coli DNA contamination | 500 U | <10 copies |
| RNase contamination | 500 U | No detectable non-specific RNase |