500 U (evaluation pack) T4 RNA Ligase 2 Truncated (5000 U/ml) and 10X T4 RNA Ligase 2, Truncated Buffer (1 x 1.5 ml).
The T4 RNA Ligase 2, Truncated is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
Features
- Specifically joins the pre-adenylated 5´ end of DNA or RNA to the 3´ end of RNA.
- In contrast to the full-length ligase, it is unable to join the phosphorylated 5´ end of RNA or DNA to the 3´ end of RNA.
- Ligation does not require ATP, but pre-adenylated substrate is needed.
- Minimizes background ligation by utilizing only pre-adenylated linkers.
Product Details
T4 RNA Ligase 2 Truncated catalyzes the phosphodiester bond formation between a pre-adenylated 5’ phosphate (DNA or RNA) and the 3’ hydroxyl of RNA. The truncated enzyme only contains the first 249 amino acids of the full-length T4 RNA Ligase 2, which makes the enzyme require a pre-adenylated 5’ terminal donor and eliminates the need for ATP. Because T4 RNA ligase 2 truncated cannot use the 5’ phosphate of RNA or DNA as a donor in the ligation reaction, it is useful for certain applications such as linker ligations with pre-adenylated 5’ DNA to 3’ hydroxyl RNA. The desired specific ligation products are enhanced dramatically over unwanted background ligation products, making the truncated enzyme superior to the full-length enzyme for this use. Thus, this enzyme is an excellent choice for RNA library preparation for RNA sequencing (1-5).
Supplied in:
10 mM Tris-HCl, 100 mM NaCl, 0.1 mM DTT, 0.1 mM EDTA, 50% glycerol (pH 7.5 at 25°C)
Supplied with:
10X T4 RNA Ligase 2, Truncated Buffer (B6070): 500mM Tris-HCl, 100mM MgCl2, 50mM DTT (pH 7.6 @ 25⁰C)
Performance
Properties:
Storage temperature: –25°C to –15°C
| Test | Specifications |
| SDS Purity | >99% |
| Specific Activity | 30,000 U/mg |
| Single-stranded exonuclease | <5.0% Released |
| Double-stranded exonuclease | <1.0% Released |
| Double-stranded endonuclease | No conversion |
| E. coli DNA contamination | <10 copies |
| Non-specific RNase | No detectable non-specific RNase |