Taq DNA Ligase (40,000 U/mL) and 10X Taq DNA Ligase Buffer
The Taq DNA Ligase (20,000 U) is intended for molecular biology applications. This product is neither intended for the diagnosis, prevention or treatment of a disease, nor has it been validated for such use either alone or in combination with other products.
Features
- Thermostable DNA Ligase
- Seals nicks
- Discriminates against mismatch ligation
Product Details
Taq DNA Ligase catalyzes the formation of a phosphodiester bond in duplex DNA containing adjacent 5'-phosphoryl and 3'-hydroxyl termini, using NAD+ as a cofactor (1).
Supplied in:
10 mM Tris-HCl, 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, 0.1% Tween-20, 50% glycerol (pH 7.5 at 25°C)
Supplied with:
10X Taq DNA Ligase Buffer (B6060): 200 mM Tris-HCl, 250 mM KCl, 100 mM MgCl2, 5 mM NAD+, 0.1% Triton X-100 (pH 7.6 at 25°C)
Performance
- Storage temperature: –25°C to –15°C
- Molecular weight: 76.9 kDa
| Test | Amount tested | Specification |
|---|
| Purity | n/a | >99% |
| Specific activity | n/a | 400,000 U/mg |
| Single-stranded exonuclease | 400 U | <5.0 % released |
| Double-stranded exonuclease | 400 U | <1.0 % released |
| Double-stranded endonuclease | 400 U | No conversion |
| E. coli DNA contamination | 400 U | <10 copies |