250 U of StableScript Reverse Transcriptase (0.05 mL at 5000 U/mL) and 4x StableScript Reaction Buffer (1 x 1.5 mL)
The StableScript Reverse Transcriptase is intended for molecular biology applications. This product is neither intended for the diagnosis, prevention or treatment of a disease, nor has it been validated for such use either alone or in combination with other products.
Features
- Versatile reverse transcriptase suitable for RT-PCR and one-step-RT-qPCR
- Recommended for long-range RT-PCR
- Highly sensitive RNA detection
- Improved thermostability, processivity and inhibitor resistance
Product Details
StableScript is a versatile reverse transcriptase designed for use in one-step RT-qPCR and long-range RT-PCR. StableScript reverse transcriptase has no detectable RNase H activity and demonstrates increased thermostability, performing well at a temperature range of 45° to 65°C. This helps to overcome the detection challenges of difficult RNA targets and enables high cDNA yields. StableScript reverse transcriptase shows reliable performance in the presence of common inhibitors.
One unit of StableScript Reverse Transcriptase is defined as the amount of enzyme required to incorporate 1 nmol of dTTP into acid insoluble material in 30 minutes at 55°C using poly r(A)/oligo(dT) as a substrate.
The enzyme is supplied in 20 mM KPO4, 1 mM EDTA, 1 mM DTT, stabilizer, 50% glycerol; pH 7.0 at 25°C.
The enzyme is supplied with a 4X StableScript Reaction Buffer.
Performance
Polymerase properties
- Optimum extension temperature: 55°C
- Transcript length: Up to 12.3 kb
| Test | Specification |
| Purity | >99% |
| Single-stranded exonuclease | 5000 U <5% released |
| Double-stranded exonuclease | 5000 U <1% released |
| Double-stranded endonuclease | 5000 U = No conversion |
| E. coli DNA contamination | 5000 U <10 copies |
| RNase contamination | 5000 U; No detectable non-specific RNase |
| Functional RT-PCR assay | Amplification of test lot within 1 Ct of
reference lot in a one-step RT- qPCR assay |