5000 U of DNA Polymerase I (10,000 U/mL) and 10x Blue Buffer. Storage temperature: –25°C to –15°C
The DNA Polymerase I (5000 U) is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
The DNA Polymerase I is intended for molecular biology applications. This product is neither intended for the diagnosis, prevention or treatment of a disease, nor has it been validated for such use either alone or in combination with other products.
Features
- Synthesizes DNA in 5′-3′ direction
- Possesses both 3′→5′ and 5′→3′ exonuclease activities
- Enables nick-translation during DNA synthesis
Product Details
DNA Polymerase I is a mesophilic DNA polymerase that exhibits 5’-3’ DNA synthesis, in addition to both 3′→5′ and 5′→3′ exonuclease activities. The combination of DNA synthesis and 5′→3′ nuclease characteristics enable nick-translation during DNA synthesis.
The protein is produced by a recombinant E. coli strain carrying the PolA gene.
The enzyme is supplied in 25 mM Tris-HCl 0.1 mM EDTA 1.0 mM dithiothreitol 50% glycerol pH 7.4 at 25°C.
It is supplied with a 10X Blue Buffer (B0110) containing: 500 mM NaCl, 100 mM Tris-HCl, 100 mM MgCl2, 10 mM DTT, pH 7.9 at 25°C.
Performance
| Assay | Specification |
| Purity | >99% |
| Specific activity | 6850 U/mg |
| Single-stranded exonuclease | Functional |
| Double-stranded exonuclease | Functional |
| Double-stranded endonuclease | 200 U; no conversion |
| E. coli DNA contamination | 200 U; <10 copies |