20,000 U/mL and 10X T4 RNA Ligase Buffer (1 x 1.5 mL)
The T4 RNA Ligase 1 (10,000 U) is intended for molecular biology applications. This product is neither intended for the diagnosis, prevention or treatment of a disease, nor has it been validated for such use either alone or in combination with other products.\
Features
- ATP-dependent ligation of single-stranded RNA and single-stranded DNA
Product Details
T4 RNA Ligase catalyzes the ATP-dependent ligation of single-stranded nucleic acids (RNA or DNA) by joining a 5' phosphoryl-terminated nucleic acid donor to a 3' hydroxyl-terminated nucleic acid acceptor through the formation of a 3'→5' phosphodiester bond (1).
Supplied in:
10 mM Tris-HCl, 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, 50% glycerol (pH 7.5 at 25°C)
Supplied with:
10X T4 RNA Ligase Buffer (B6050): 500 mM Tris-HCl, 100 mM MgCl2, 10 mM ATP, 100 mM DTT (pH 7.8 at 25°C)
- Storage temperature: –25°C to –15°C
- Molecular weight: 43.5 kDa
| Test | Amount tested | Specification |
|---|
| Purity | n/a | >99% |
| Specific activity | n/a | 16,800 U/mg |
| Single-stranded exonuclease | 200 U | <5.0 % released |
| Double-stranded exonuclease | 200 U | <1.0 % released |
| Double-stranded endonuclease | 200 U | No conversion |
| E. coli DNA contamination | 200 U | <10 copies |
| RNase contamination | 200 U | No detectable non-specific RNase |