The T4 DNA Ligase is intended for molecular biology applications. This product is neither intended for the diagnosis, prevention or treatment of a disease, nor has it been validated for such use either alone or in combination with other products.
Features
- Versatile enzyme widely used for high-precision NGS library prep and DNA cloning
- Efficiently joins both complementary cohesive (sticky) ends and blunt ends
- Repairs single-stranded nicks in duplex DNA for high-efficiency DNA ligation
- High-concentration rapid formulation increases efficiency and decreases ligation times
- Quality tested for the absence of DNase contamination
Product Details
T4 DNA Ligase enzyme is a recombinant protein (55,292 Daltons) produced by an E. coli strain carrying the cloned bacteriophage T4 DNA ligase gene.
T4 DNA Ligase is an ATP-dependent enzyme that catalyzes the formation of a phosphodiester bond between the terminal 5ʹ phosphate and a 3' hydroxyl group of duplex DNA or RNA. The enzyme efficiently joins blunt and cohesive ends and repairs single-stranded nicks in duplex DNA, RNA or DNA:RNA hybrids (1). T4 DNA Ligase is effective for joining blunt or cohesive ends in double-stranded RNA with a complementary "splint" strand (DNA or RNA) holding the RNA ends together. T4 DNA Ligase cannot join single-stranded nucleic acids.
Available Configurations:
| Feature | T4 DNA Ligase (Standard) | T4 DNA Ligase (Rapid) | WGS Ligase |
|---|
| Catalog number | L6030-LC-L | L6030-HC-L | L6030-W-L |
| Best for | Routine cloning and ligation of cohesive or blunt DNA ends | Fast ligation and improved blunt-end efficiency | Whole genome sequencing (WGS) library construction and adapter ligation |
| Ligase concentration | 120,000 U/mL | 600,000 U/mL | 600,000 U/mL |
Buffers included
(PEG concentration) | 10x T4 DNA Ligase Buffer | 10x T4 DNA Ligase Buffer
2x Rapid Ligation Buffer (15% PEG 6000) | 5x Rapid Ligation Buffer (30% PEG 6000) |
| Ligation time | 30 minutes at 25°C | 10 minutes at 25°C | 15 minutes at 20°C |
Product Composition:
| Component | Composition | Storage temperature |
|---|
| T4 DNA Ligase (L6030) | Supplied in 10 mM Tris-HCl, 50 mM KCl, 1 mM DTT, 0.1 mM EDTA and 50% glycerol; pH 7.5 at 25°C. | –25°C to –15°C |
| 10x T4 DNA Ligase Buffer (B6030) | 500 mM Tris-HCI, 100 mM MgCl2, 50 mM DTT and 10 mM ATP; pH 7.6 at 25°C. | –25°C to –15°C |
| 2x Rapid Ligation Buffer (B1010) | 132 mM Tris-HCl, 20 mM MgCl2, 2 mM DTT, 2 mM ATP and 15% PEG 6000; pH 7.6 at 25°C. | –25°C to –15°C |
| 5x Rapid Ligation Buffer (B9020) | 330 mM Tris-HCl, 50mM MgCl2 , 5 mM DTT, 5 mM ATP and 30% PEG 6000; pH 7.6 @ 25°C | –25°C to –15°C |