500 U of Thermolabile UDG (0.5 mL at 1000 U/mL) and 10x Thermolabile UDG Reaction Buffer (2 x 1.5 mL).
The Thermolabile UDG is intended for molecular biology applications. This product is neither intended for the diagnosis, prevention or treatment of a disease, nor has it been validated for such use either alone or in combination with other products.
Features
- Removes uracil from DNA, leaving an AP site
- Inactivated by a 10-minute incubation at >50°C
- No activity on RNA substrates
Product Details
Thermolabile UDG removes uracil from DNA by hydrolyzing the N-glycosylic bond between the deoxyribose and the base, leaving an apurinic or apyrimidinic site. There is no activity shown on RNA substrates, making it usable for RT-PCR. This enzyme (1–10 units) is completely inactivated by a 10-minute incubation at temperatures greater than 50°C in the 1x reaction buffer as measured in the unit characterization assay.
This enzyme is supplied in 50 mM Tris-HCl, 50 mM NaCl, 1 mM DTT, 0.1 mM EDTA and 50% glycerol; pH 7.5 at 25°C.
The 10x Reaction Buffer contains 700 mM Tris-HCl, 100 mM NaCl, 10 mM EDTA and 1 mg/mL BSA; pH 8 at 25°C.
Performance
- Storage temperature: –25°C to –15°C
- Molecular weight: 26,218 Daltons
| Test | Amount tested | Specification |
|---|
| Purity | n/a | >98% |
| Specific activity | n/a | 30,000 U/mg |
| Single-stranded exonuclease | 10 units | <1% released |
| Double-stranded exonuclease | 10 units | <1% released |
| Double-stranded endonuclease | 5 units | No conversion |
| E. coli DNA contamination | 5 units | <10 copies |