Uracil DNA Glycosylase (5.0 mL at 2000 U/mL) and 10x UDG Reaction Buffer (3 x 1.5 mL).
The Uracil DNA Glycosylase is intended for molecular biology applications. This product is neither intended for the diagnosis, prevention or treatment of a disease, nor has it been validated for such use either alone or in combination with other products.
Features
- Removes uracil from DNA
- Exhibits no measurable activity on short oligonucleotides (<6 bases) and RNA substrates
Product Details
Uracil-DNA Glycosylase catalyzes the hydrolysis of the N-glycosylic bond between uracil and the sugar, leaving an abasic site in uracil-containing single- or double-stranded DNA. The enzyme shows no measurable activity on short oligonucleotides (<6 bases), or RNA substrates.
This enzyme is supplied in 10 mM Tris-HCl, 50 mM NaCl, 1 mM DTT, 0.1 mM EDTA and 50% glycerol; pH 7.5 at 25°C.
The 10x UDG Reaction Buffer contains 200 mM Tris-HCI, 10 mM DTT and 10 mM EDTA; pH 8 at 25°.
Performance
- Storage temperature: –25°C to –15°C
- Molecular weight: 25,693 Daltons
| Test | Amount tested | Specification |
|---|
| Purity | n/a | >95% |
| Specific activity | n/a | 77,000 U/mg |
| Single-stranded exonuclease | 100 U | <5% released |
| Double-stranded exonuclease | 100 U | <1% released |
| Double-stranded endonuclease | 100 U | No conversion |
| E. coli DNA contamination | 100 U | <10 copies |