To extend the shelf life of the kit, components are packed in two separate boxes based on their storage requirements: Box 1 is intended for storage at 2–8°C, while Box 2 should be kept at –30 to -15°C. This separation helps maintain the stability and performance of the reagents throughout the kit's intended shelf life.
The Saltonase ELISA Kit (5 x 96) is intended for molecular biology applications. This product is neither intended for the diagnosis, prevention or treatment of a disease, nor has it been validated for such use either alone or in combination with other products.
Features
- Highly sensitive and specific using monoclonal antibodies
- The assay has a broad operating range of 31.25 to 2000 pg/mL, measured at OD450
- The detection method is colorimetric
- Provided in a practical format of 8-well strips
- Used together with Saltonase® MBG and GMP-grade, they streamline both the enzymatic digestion of nucleic acids and the detection of residual nuclease
Product Details
The Saltonase ELISA Kit (5 x 96) is a high-performance assay designed for the quantitative detection of Saltonase endonuclease, including MBG and GMP-grade formats. Engineered for precision, the assay utilizes highly specific monoclonal antibodies to ensure consistent, reliable results with a detection limit of as low as 31.25 pg/mL.
Optimized for bioprocessing applications, Saltonase is an endonuclease that removes nucleic acids under high-salt and variable pH conditions. In addition, the ELISA kit is ideal for monitoring residual Saltonase levels in in-process samples and verifying its removal in downstream processing and final product.
Each kit includes five pre-coated 96-well microplates in convenient 8-well strip formats and comes with ready-to-use reagents. To extend shelf life and maintain reagent stability, the kit components are packaged in two separate sets. The kit supports a wide quantification range of 31.25–2000 pg/mL and provides sufficient materials for up to 480 measurements.
Performance
Caption| Assay characteristics | Specification |
| Sensitivity | LOQ: 30 pg/mL |
| Broad working range | 31.25–2000 pg/mL |
| Specificity | Monoclonal Antibodies used Low interference from the sample matrix |
| Accuracy | RE +/- 20% |
| Processing time | 240 min |
| Flexibility | 8-well strip plate format |