UDGase

125972 EN19 Media/Uploads/EN19-250(4).webp UDGase <h3 _ngcontent-serverapp-c1326938488="" class="intro__subtitle ng-star-inserted" style="color: rgb(89, 89, 89); background-color: rgb(249, 249, 249); padding: 0px; font-size: 20px; font-family: &quot;Futura W01&quot;, Arial, Helvetica, sans-serif; font-weight: 500; font-stretch: normal; border: 0px; letter-spacing: 0.15px;"><span style="font-family: Tahoma; font-size: 16px;"><strong>For the catalyzation of the release of uracil from uracil-containing single-stranded or double-stranded DNA</strong></span></h3><span style="font-family: Tahoma; font-size: 16px;"><strong><wca-product-details _ngcontent-serverapp-c3223413817="" _nghost-serverapp-c3050437383="" data-onedatalayer-source="Product Details" class="ng-star-inserted" style="color: rgb(89, 89, 89); background-color: rgb(255, 255, 255); font-size: 16px; letter-spacing: 0.25px;"><wca-product-description _ngcontent-serverapp-c3050437383="" _nghost-serverapp-c1998474798="" data-onedatalayer-source="Product Description" class="ng-star-inserted"></wca-product-description></wca-product-details></strong></span><h4 _ngcontent-serverapp-c1998474798="" class="section" style="width: calc(66.6667% - 12px); padding: 0px; font-size: 28px; font-family: &quot;Futura W01&quot;, Arial, Helvetica, sans-serif; font-stretch: normal; border: 0px; letter-spacing: 0px;"><span style="font-size: 14px;"><span style="font-family: Tahoma;"><strong><span class="medium">Features</span></strong></span></span></h4><span style="font-family: Tahoma; font-size: 14px;"><wca-product-details _ngcontent-serverapp-c3223413817="" _nghost-serverapp-c3050437383="" data-onedatalayer-source="Product Details" class="ng-star-inserted" style="color: rgb(89, 89, 89); background-color: rgb(255, 255, 255); font-size: 16px; letter-spacing: 0.25px;"><wca-product-description _ngcontent-serverapp-c3050437383="" _nghost-serverapp-c1998474798="" data-onedatalayer-source="Product Description" class="ng-star-inserted"><section _ngcontent-serverapp-c1998474798="" style="padding: 0px; border: 0px;"><wca-lightbox _ngcontent-serverapp-c1998474798="" class="description-box" lg-uid="lg0" style="width: 1236px;"></wca-lightbox><div _ngcontent-serverapp-c1998474798="" wcatablegradient="" class="rich-text product-description" style="padding: 0px; font-size: 18px; border: 0px; letter-spacing: 0.5px;"><ul style="width: calc(66.6667% - 12px); padding: 0px 0px 0px 18px; border: 0px; list-style-image: initial;"><li style="color: inherit; padding: 8px 0px 0px; font-size: inherit; border: 0px; letter-spacing: inherit;">Modified recombinant enzyme over-expressed in&nbsp;<em style="padding: 0px; border: 0px;">E. coli</em></li><li style="color: inherit; padding: 8px 0px 0px; font-size: inherit; border: 0px; letter-spacing: inherit;">Sensitive mutant is irreversibly inactive after heating</li><li style="color: inherit; padding: 8px 0px 0px; font-size: inherit; border: 0px; letter-spacing: inherit;">Catalyzes the release of uracil from uracil-containing single-stranded or double-stranded DNA but not from RNA or oligonucleotides</li><li style="color: inherit; padding: 8px 0px 0px; font-size: inherit; border: 0px; letter-spacing: inherit;">Active over a broad pH range (optimal at pH 8.0)</li><li style="color: inherit; padding: 8px 0px 0px; font-size: inherit; border: 0px; letter-spacing: inherit;">Shows no activity on RNA or oligonucleotides</li></ul></div></section></wca-product-description></wca-product-details><wca-product-details _ngcontent-serverapp-c3223413817="" _nghost-serverapp-c3050437383="" data-navigation="Product Details" data-onedatalayer-source="Product Details" class="ng-star-inserted" style="color: rgb(89, 89, 89); background-color: rgb(255, 255, 255); font-size: 16px; letter-spacing: 0.25px;"><wca-product-description _ngcontent-serverapp-c3050437383="" _nghost-serverapp-c1998474798="" data-onedatalayer-source="Product Description" class="ng-star-inserted"></wca-product-description></wca-product-details></span><h4 _ngcontent-serverapp-c1998474798="" class="intro" style="width: calc(66.6667% - 12px); padding: 0px; font-size: 40px; border: 0px;"><span style="font-size: 14px;"><span style="font-family: Tahoma;" class="medium"><strong>Product Details</strong></span></span></h4><span style="font-family: Tahoma; font-size: 14px;"><wca-product-details _ngcontent-serverapp-c3223413817="" _nghost-serverapp-c3050437383="" data-navigation="Product Details" data-onedatalayer-source="Product Details" class="ng-star-inserted" style="color: rgb(89, 89, 89); background-color: rgb(255, 255, 255); font-size: 16px; letter-spacing: 0.25px;"><wca-product-description _ngcontent-serverapp-c3050437383="" _nghost-serverapp-c1998474798="" data-onedatalayer-source="Product Description" class="ng-star-inserted"><section _ngcontent-serverapp-c1998474798="" style="padding: 0px; border: 0px;"><span class="medium"><strong><wca-lightbox _ngcontent-serverapp-c1998474798="" class="description-box" lg-uid="lg1" style="width: 1236px;"></wca-lightbox></strong></span><div _ngcontent-serverapp-c1998474798="" wcatablegradient="" class="rich-text product-description" style="padding: 0px; font-size: 18px; border: 0px; letter-spacing: 0.5px;"><p style="color: inherit; width: calc(66.6667% - 12px); padding: 0px; font-size: inherit; border: 0px; letter-spacing: inherit;">UDGase catalyzes the hydrolysis of the N-glycosylic bond between uracil and sugar, leaving an apyrimidinic site in uracil-containing single-stranded or double-stranded DNA. The enzyme shows no activity on RNA or oligonucleotides.</p><p style="color: inherit; width: calc(66.6667% - 12px); padding: 0px; font-size: inherit; border: 0px; letter-spacing: inherit;"><br></p><p style="color: inherit; width: calc(66.6667% - 12px); padding: 0px; font-size: inherit; border: 0px; letter-spacing: inherit;">It is supplied with 20 mM Tris-Cl, pH 8.0, 0.1 M NaCl, 0.1 mM EDTA , 1 mM DTT, 0.1% stabilizer and 50% glycerol.</p><p style="color: inherit; width: calc(66.6667% - 12px); padding: 0px; font-size: inherit; border: 0px; letter-spacing: inherit;"><br></p><p style="color: inherit; width: calc(66.6667% - 12px); padding: 0px; font-size: inherit; border: 0px; letter-spacing: inherit;">One unit is defined as the amount of enzyme that catalyzes the release of 60 pmol of uracil per minute from uracil-containing dsDNA. The activity is measured by the release of [3H]-uracil in a 50 μL reaction containing 0.2 μg DNA in 30 minutes at 37°C.</p><h4 _ngcontent-serverapp-c1998474798="" class="section" style="width: calc(66.6667% - 12px); padding: 0px; font-size: 28px; font-family: &quot;Futura W01&quot;, Arial, Helvetica, sans-serif; font-stretch: normal; border: 0px; letter-spacing: 0px;"><span style="font-family: Tahoma;" class="medium"><strong>Principle</strong></span></h4><p style="color: inherit; width: calc(66.6667% - 12px); padding: 0px; font-size: inherit; border: 0px; letter-spacing: inherit;"><wca-product-details _ngcontent-serverapp-c3223413817="" _nghost-serverapp-c3050437383="" data-onedatalayer-source="Product Details" class="ng-star-inserted" style="color: rgb(89, 89, 89); background-color: rgb(255, 255, 255); font-size: 16px; font-family: &quot;Futura W01&quot;, Arial, Helvetica, sans-serif; letter-spacing: 0.25px;"><wca-product-description _ngcontent-serverapp-c3050437383="" _nghost-serverapp-c1998474798="" data-onedatalayer-source="Product Description" class="ng-star-inserted"></wca-product-description></wca-product-details></p><section _ngcontent-serverapp-c1998474798="" style="padding: 0px; border: 0px;"><wca-lightbox _ngcontent-serverapp-c1998474798="" class="description-box" lg-uid="lg3" style="width: 1236px;"></wca-lightbox><div _ngcontent-serverapp-c1998474798="" wcatablegradient="" class="rich-text product-description" style="padding: 0px; font-size: 18px; border: 0px; letter-spacing: 0.5px;"><p style="color: inherit; width: calc(66.6667% - 12px); padding: 0px; font-size: inherit; border: 0px; letter-spacing: inherit;"><em style="padding: 0px; border: 0px;">E. coli</em>&nbsp;Uracil DNA Glycosylase catalyzes the hydrolysis of the N-glycosylic bond between uracil and sugar, leaving an apyrimidinic site in uracil-containing single-stranded or double-stranded DNA. The enzyme shows no activity on RNA or oligonucleotides.</p></div></section><wca-product-details _ngcontent-serverapp-c3223413817="" _nghost-serverapp-c3050437383="" data-onedatalayer-source="Product Details" class="ng-star-inserted" style="color: rgb(89, 89, 89); background-color: rgb(255, 255, 255); font-size: 16px; font-family: &quot;Futura W01&quot;, Arial, Helvetica, sans-serif; letter-spacing: 0.25px;"><wca-product-description _ngcontent-serverapp-c3050437383="" _nghost-serverapp-c1998474798="" data-onedatalayer-source="Product Description" class="ng-star-inserted"></wca-product-description></wca-product-details><p><br></p><h4 _ngcontent-serverapp-c1998474798="" class="section" style="width: calc(66.6667% - 12px); padding: 0px; font-size: 28px; font-family: &quot;Futura W01&quot;, Arial, Helvetica, sans-serif; font-stretch: normal; border: 0px; letter-spacing: 0px;"><span class="medium"><strong>Procedure</strong></span></h4><p style="color: inherit; width: calc(66.6667% - 12px); padding: 0px; font-size: inherit; border: 0px; letter-spacing: inherit;"><wca-product-details _ngcontent-serverapp-c3223413817="" _nghost-serverapp-c3050437383="" data-onedatalayer-source="Product Details" class="ng-star-inserted" style="color: rgb(89, 89, 89); background-color: rgb(255, 255, 255); font-size: 16px; font-family: &quot;Futura W01&quot;, Arial, Helvetica, sans-serif; letter-spacing: 0.25px;"><wca-product-description _ngcontent-serverapp-c3050437383="" _nghost-serverapp-c1998474798="" data-onedatalayer-source="Product Description" class="ng-star-inserted"></wca-product-description></wca-product-details></p><section _ngcontent-serverapp-c1998474798="" style="padding: 0px; border: 0px;"><wca-lightbox _ngcontent-serverapp-c1998474798="" class="description-box" lg-uid="lg4" style="width: 1236px;"></wca-lightbox><div _ngcontent-serverapp-c1998474798="" wcatablegradient="" class="rich-text product-description" style="padding: 0px; font-size: 18px; border: 0px; letter-spacing: 0.5px;"><p style="color: inherit; width: calc(66.6667% - 12px); padding: 0px; font-size: inherit; border: 0px; letter-spacing: inherit;"><span style="padding: 0px; font-weight: 700; border: 0px;">Quality Control</span></p><p style="color: inherit; width: calc(66.6667% - 12px); padding: 0px; font-size: inherit; border: 0px; letter-spacing: inherit;">UDGase is tested for degradation of PCR product containing dUTPs. The product is free of unspecific DNA nucleases.</p><p style="color: inherit; width: calc(66.6667% - 12px); padding: 0px; font-size: inherit; border: 0px; letter-spacing: inherit;"><br></p><p style="color: inherit; width: calc(66.6667% - 12px); padding: 0px; font-size: inherit; border: 0px; letter-spacing: inherit;"><span style="padding: 0px; font-weight: 700; border: 0px;">Usage</span></p><p style="color: inherit; width: calc(66.6667% - 12px); padding: 0px; font-size: inherit; border: 0px; letter-spacing: inherit;">Treatment of 0.1 µg of uracil-containing DNA with 1 unit of UDG for 10 minutes at 37°C renders the DNA incapable of being copied by DNA polymerase. The enzyme can be irreversibly inactivated by incubating at 95°C for 10 minutes.</p></div></section><wca-product-details _ngcontent-serverapp-c3223413817="" _nghost-serverapp-c3050437383="" data-onedatalayer-source="Product Details" class="ng-star-inserted" style="color: rgb(89, 89, 89); background-color: rgb(255, 255, 255); font-size: 16px; font-family: &quot;Futura W01&quot;, Arial, Helvetica, sans-serif; letter-spacing: 0.25px;"><wca-product-description _ngcontent-serverapp-c3050437383="" _nghost-serverapp-c1998474798="" data-onedatalayer-source="Product Description" class="ng-star-inserted"></wca-product-description></wca-product-details><p><br></p><h4 _ngcontent-serverapp-c1998474798="" class="section" style="width: calc(66.6667% - 12px); padding: 0px; font-size: 28px; font-family: &quot;Futura W01&quot;, Arial, Helvetica, sans-serif; font-stretch: normal; border: 0px; letter-spacing: 0px;">Applications</h4><p style="color: inherit; width: calc(66.6667% - 12px); padding: 0px; font-size: inherit; border: 0px; letter-spacing: inherit;"><wca-product-details _ngcontent-serverapp-c3223413817="" _nghost-serverapp-c3050437383="" data-onedatalayer-source="Product Details" class="ng-star-inserted" style="color: rgb(89, 89, 89); background-color: rgb(255, 255, 255); font-size: 16px; font-family: &quot;Futura W01&quot;, Arial, Helvetica, sans-serif; letter-spacing: 0.25px;"><wca-product-description _ngcontent-serverapp-c3050437383="" _nghost-serverapp-c1998474798="" data-onedatalayer-source="Product Description" class="ng-star-inserted"></wca-product-description></wca-product-details></p><section _ngcontent-serverapp-c1998474798="" style="padding: 0px; border: 0px;"><wca-lightbox _ngcontent-serverapp-c1998474798="" class="description-box" lg-uid="lg5" style="width: 1236px;"></wca-lightbox><div _ngcontent-serverapp-c1998474798="" wcatablegradient="" class="rich-text product-description" style="padding: 0px; font-size: 18px; border: 0px; letter-spacing: 0.5px;"><p style="color: inherit; width: calc(66.6667% - 12px); padding: 0px; font-size: inherit; border: 0px; letter-spacing: inherit;">This is used for applications such as:</p><ul style="width: calc(66.6667% - 12px); padding: 0px 0px 0px 18px; border: 0px; list-style-image: initial;"><li style="color: inherit; padding: 8px 0px 0px; font-size: inherit; border: 0px; letter-spacing: inherit;">Elimination of carry-over contamination in PCR</li><li style="color: inherit; padding: 8px 0px 0px; font-size: inherit; border: 0px; letter-spacing: inherit;">Probe for protein-DNA interaction studies</li><li style="color: inherit; padding: 8px 0px 0px; font-size: inherit; border: 0px; letter-spacing: inherit;">Glycosylase mediated single nucleotide polymorphism detection (GMPD)</li><li style="color: inherit; padding: 8px 0px 0px; font-size: inherit; border: 0px; letter-spacing: inherit;">Molecular cloning of PCR products</li></ul></div></section><p><br></p></div></section></wca-product-description></wca-product-details></span><br> 843.38 714.73 page_30079 0 0 0