Image blots hours after substrate incubation. A slot blot containing a serial dilution of an HRP-conjugated antibody was incubated with Radiance Plus and imaged at the times indicated. After 8 hours, the bands are still easily detected with only a 2-minute exposure.Reference Publications
Kelly Ascençao, Nahzli Dilek, Fiona Augsburger, Theodora Panagaki, Karim Zuhra, Csaba Szabo
Pharmacological induction of mesenchymal-epithelial transition via inhibition of H2S biosynthesis and consequent suppression of ACLY activity in colon cancer cells
Pharmacological Research 165 (2021) 105393
DOI: https://doi.org/10.1016/j.phrs.2020.105393
Article Snippet: “After being washed 3 times with TBST, the blots were developed with Radiance Plus (AC2103, Azure Biosystems, Dublin, CA, USA) chemiluminescence solutions using Azure Imaging System 300 (Azure Biosystems) and quantified using the ImageJ software (NIH, Bethesda, MD, USA).”
Begg, Lauren R et al.
S100A8/A9 predicts response to PIM kinase and PD-1/PD-L1 inhibition in triple-negative breast cancer mouse models
Communications medicine vol. 4,1 22
DOI: https://doi.org/10.1038%2Fs43856-024-00444-8
Article Snippet: “ The ECL reaction was done using Radiance Plus Chemiluminescent Substrate (Azure Biosystems, AC2103), and chemiluminescent signals were acquired with the Bio-Rad ChemiDoc Touch Imaging system equipped with a supersensitive CCD camera.”
Traxler D, Dannenberg V, Zlabinger K, et al.
Plasma Small Extracellular Vesicle Cardiac miRNA Expression in Patients with Ischemic Heart Failure, Randomized to Percutaneous Intramyocardial Treatment of Adipose Derived Stem Cells or Placebo: Subanalysis of the SCIENCE Study
International Journal of Molecular Sciences. 2023; 24(13):10647.
DOI: https://doi.org/10.3390/ijms241310647
Article Snippet: “Incubation with secondary antibody (ab6721, Abcam, Cambridge, GBR) in 2% milk in TBST was performed for 1 h at room temperature. Chemiluminescence visualization was performed with Radiance Plus (AC2103, Azure Biosystems, Dublin, CA, USA) at an Azure c600 (Azure Biosystems, Dublin, CA, USA).”
Stofkova A, Zloh M, Andreanska D, et al.
Depletion of Retinal Dopaminergic Activity in a Mouse Model of Rod Dysfunction Exacerbates Experimental Autoimmune Uveoretinitis: A Role for the Gateway Reflex
International Journal of Molecular Sciences. 2022; 23(1):453.
DOI: https://doi.org/10.3390/ijms23010453
Article Snippet: “Chemiluminescence was achieved by applying Radiance Plus Substrate with Radiance Peroxide (AC2103; Azure Biosystems, Dublin, CA, USA) to the membranes.”
Martins V, Santos SS, Rodrigues LdOCP, Salomao R, Liaudet L, Szabo C.
Efficacy of Clinically Used PARP Inhibitors in a Murine Model of Acute Lung Injury
Cells. 2022; 11(23):3789.
DOI: https://doi.org/10.3390/cells11233789
Article Snippet: “Immunoreactive proteins were visualized by Radiance Plus (AC2103, Azure Biosystems, Dublin, CA, USA) chemiluminescence solution using an Azure Imaging System 300 (Azure Biosystems).”