Long-lasting signal. Radiance allows blots to be imaged several hours after substrate incubation. Blots can be re-imaged to obtain the perfect exposure, without worrying about signal decay. A blot was imaged with 2 min exposures at 5 min and 60 min after Reference Publications
Rohm M, Volke L, Schlaffke L, Rehmann R, Südkamp N, Roos A, Schänzer A, Hentschel A, Vorgerd M.
Dysregulation of Metabolism and Proteostasis in Skeletal Muscle of a Presymptomatic Pompe Mouse Model
Cells. 2023; 12(12):1602.
DOI: https://doi.org/10.3390/cells12121602
Article Snippet: “Antibody binding was detected by chemiluminescent substrate Radiance Q (AC2101, Azure Biosystems, Dublin, CA, USA) in the c600 Imaging System (Azure Biosystems, Dublin, CA, USA). The optical densities (OD) of the Coomassie staining were quantified using the Azure Biosystems software.”
Williams, C.H., Neitzel, L.R., Cornell, J. et al.
GPR68-ATF4 signaling is a novel prosurvival pathway in glioblastoma activated by acidic extracellular microenvironment
Exp Hematol Oncol 13, 13 (2024).
DOI: https://doi.org/10.1186/s40164-023-00468-1
Article Snippet: “The membranes were washed with PBST in four, five-minute intervals before protein visualization using Radiance Q (CAT # AC2101, Azure Biosystems, Dublin, CA, USA) on Bio-Rad ChemiDoc TM MP Imaging System (Hercules, CA, USA).”
Skowicki, Michal et al.
FAP Targeting of Photosensitizer-Loaded Polymersomes for Increased Light-Activated Cell Killing
Biomacromolecules vol. 25,2 (2024): 754-766.
DOI: 10.1021/acs.biomac.3c00943
Article Snippet: “Membranes were washed 4 times with PBS-T, incubated for 30 s in Radiance Q-HRP chemiluminescence substrate (#AC2101, Azure), and imaged using a ChemiDoc MP imaging system.”
Korade, Zeljka et al.
Chronic Aripiprazole and Trazodone Polypharmacy Effects on Systemic and Brain Cholesterol Biosynthesis
Biomolecules vol. 13,9 1321.
DOI: 10.3390/biom13091321
Article Snippet: “After three washes, the membranes were incubated with the secondary antibodies for 1 h at RT. After extensive washing, the blots were exposed to a chemiluminescence reagent (Radiance Q, Azure Biosystems AC2101) and visualized on an Azure 300 instrument (Azure Biosystems, Dublin, CA, USA).”
Catherine Rippe
Examining the Immune Regulation of NLRP12 Through Novel Protein Interactions
MSU Graduate Theses. 3837.
DOI: https://bearworks.missouristate.edu/theses/3837
Article Snippet: “The immunoblots were developed using chemiluminescence (Radiance Q, Cat. AC2101, Azure Biosystems) and visualized with an Azure C300 digital imager.”
Nguyen TGVargas-Blanco DA, Roberts LA, Shell SS.
The Impact of Leadered and Leaderless Gene Structures on Translation Efficiency, Transcript Stability, and Predicted Transcription Rates in Mycobacterium smegmatis
J Bacteriol 202:10.1128/jb.00746-19.
DOI: https://doi.org/10.1128/jb.00746-19
Article Snippet: “The membrane was rinsed as previously described and incubated with horseradish peroxidase (HRP) substrate (Radiance Q; Azure Biosystems; catalog number AC2101) as recommended by the manufacturer. Imaging was done using an Azure C200 imaging system (Azure Biosystems).”