2×HieffTM Ultra-Rapid II HotStart PCR Master Mix contains heat-stabilized Taq DNA Polymerase modified with antibodies, adds strong elongation factor and optimized buffer system, and has super high amplification efficiency. It is very suitable for PCR amplification of most colonies such as E. coli, Agrobacterium, and yeast. The extension speed of amplification of complex templates within 3 kb can reach 1 sec/kb, 3-6 kb to 3 sec/kb, 6-10 kb to 5 sec/kb and 10 kb to 10 sec/kb; the amplification speed of simple templates such as plasmid within 6 kb can reach 1 sec/kb, which can greatly save PCR reaction time. Meanwhile, Mix contains dNTPs, Mg2+, and it can be amplified only with adding primers and template. In addition, Mix contains red tracer dye, which can be used by electrophoresis directly after the end of the reaction. The protective agent added to the system enables the product to maintain a stable activity after repeated freezing and thawing. The 3′ end of the PCR product bands A and can be easily cloned into the T vector.
Rapid PCR: 1 sec/kb
High GC tolerant
Dye Plus
Long PCR
Colony Direct PCR
Genotyping
Specifications
Product specification | Master Mix |
Concentration | 2× |
Hot Start | Built-in Hot Start |
Overhang | 3'-A |
Reaction speed | Rapid |
Size (Final Product) | Up to 15 kb |
Conditions for transportation | Dry ice |
Figures
1. Nest PCR
2. Crispr Genotyping
Cited from: Viruses 2024, 16(9), 1382;
3. Colony Direct PCR
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Fig 1. Fast Colony Direct PCR with E. coli and Agrobacterium samples using 4 different speed of 1: 1s/kb, 2: 3s/kb, 3: 5s/kb and 4: 10s/kb. M: 10,000 DNA Marker (10505ES).
4. Long PCR with Genomic DNA
Fig. 2. Fast amplification of 1-10 kb mouse gDNA shows high yield and a high speed at 10 sec/kb. M: 10,000 DNA Marker (10505ES).
5. High Stability 
Storage
The products should be stored at -25~-15℃ for 2 years.
10167ES 2×Hieff® Ultra-Rapid II HotStart PCR Master Mix-Ver.EN20240517.pdf
Citations
[1] Rapid, sensitive, and visual detection of mandarin fish ranavirus and infectious spleen and kidney necrosis virus using an RPA-CRISPR/Cas12a system，Front. Microbiol. , 13 December 2024, Sec. Virology, Volume 15, 2024
[2] CRISPR/Cas9-Mediated Resistance to Wheat Dwarf Virus in Hexaploid Wheat (Triticum aestivum L.)Viruses2024, 16(9), 1382;