Description
easy-BLUE™ Total RNA Extraction Kit is a solution type that can extract total RNA easily and quickly from solid samples such as cell, tissue, and plant etc.
This kit is the product that can most efficiently extract the RNA from tissues or cells. It is theoretically possible to extract pure RNA with almost no contamination of protein and genomic DNA when the amount of RNA extraction sample is appropriate.
Characteristics
• Total RNA extraction from solid samples such as cell, tissue and plant etc
• Easy separation of aqueous phase/ phenol phase with deep-cyan blue color solution
• High efficiency and purity total RNA extraction using high purity water-saturated phenol
• Optimized protocol for each specimen
• Completely eliminates cross-contaminants for precise downstream applicationsApplication
• cDNA synthesis
• RT-PCR
• Real-time RT-PCR
• Northern blotting
• Primer extension
• Infectious disease research
Kit Contents
| No. | Kit Contents | Unit |
|---|
| 1 | easy-BLUE™ Solution | 100ml × 1 ea |
| 2 | Manual | 1 ea |
Storage
• Store in the dark at 4ºC.
Technical Data
1. Total RNA extraction from various tissues and cells
• The easy-BLUE™ Total RNA Extraction Kit provides a simple and rapid method for the isolation of total RNA from cultured cells, tissues, plants and numerous other cell sources.
• RNA was extracted from various cells and tissues using the easy-BLUE™ Total RNA Extraction Kit.
• As results, it was confirmed that high-purity RNA could be extracted.
2. RT-PCR Amplification
Evaluation of the specificity of the e-Myco™ VALiD-Q Real-time PCR Kit (ver. 2.0) for Mycoplasma related strains.
• Total RNA was purified from different tissues and cells using easy-BLUE™ Total RNA Extraction Kit.
• And then, one step RT-PCR reaction performed for β-actin (400bp) gene from RNA using ONE-STEP RT-PCR PreMix Kit (Cat. No. 25101), respectively.
3. Comparison of total RNA yield from different company kit
• RNA was extracted from various cells and tissues using the easy-BLUE ™ Total RNA Extraction Kit.
• As results, its extraction efficiency showed equal to or better than other products.
Citation List
