BIOTAQ™ is a purified thermostable DNA polymerase offering high yield over a wide range of PCR templates, and is a good choice for routine assays. BIOTAQ is a robust preparation and delivers high yields with minimal background. BIOTAQ possesses 5´-3´ exonuclease activity and leaves an ´A´ overhang such that the PCR product is suitable for effective integration into TA cloning vectors. BIOTAQ is supplied with 10x NH4-based Reaction Buffer, which provides optimal conditions for most experiments. Additional MgCl2 is provided to allow reaction conditions to be adjusted to suit the template.
Publications References:
- An efficient method to assemble linear DNA templates for in vitro screening and selection systems
Nucleic Acids Research, 2009.
https://pmc.ncbi.nlm.nih.gov/articles/PMC2764453/ (PMC) - Roles of intracellular hydrogen peroxide accumulation in abscisic acid signaling in Arabidopsis guard cells
Plant Cell Reports, 2014.
https://pmc.ncbi.nlm.nih.gov/articles/PMC4073789/ (PMC) - Development of Capsicum EST–SSR markers for species identification and in silico mapping onto the tomato genome sequence
Molecular Breeding, 2013.
https://link.springer.com/article/10.1007/s11032-012-9774-z (Springer Nature Link) - Reverse evolution leads to genotypic incompatibility despite functional and active site convergence
eLife, 2015.
https://elifesciences.org/articles/06492 (eLife) - Green light irradiation during sex differentiation induces female-to-male sex reversal in the medaka Oryzias latipes
Scientific Reports, 2019.
https://www.nature.com/articles/s41598-019-38908-w (Nature) - A Novel Connexin 30 Mutation in Clouston Syndrome
Journal of Investigative Dermatology, 2006.
https://www.sciencedirect.com/science/article/pii/S0022202X15415982 (ScienceDirect) - Paternal high-fat diet affects weight and DNA methylation of their offspring
Scientific Reports, 2024.
https://www.nature.com/articles/s41598-024-70438-y (Nature) - Antarctic fungi: a bio-source alternative to produce polyunsaturated fatty acids (PUFAs)
Scientific Reports, 2025.
https://pmc.ncbi.nlm.nih.gov/articles/PMC12889145/ (PMC) - Discriminatory Ability of Hypervariable Variable Number Tandem Repeat Loci in Population-based Analysis of Mycobacterium tuberculosis Strains, London, UK
Emerging Infectious Diseases, 2010.
https://pmc.ncbi.nlm.nih.gov/articles/PMC2866407/ (PMC) - The GRAS Protein SCL14 and TGA Transcription Factors Regulate Stress-Responsive Gene Expression
(PhD research publication record, University repository).
https://citeseerx.ist.psu.edu/document?doi=545a5bac1f9bcf7fd5a0f156e1a485b7e6fc4d5c&repid=rep1&type=pdf (citeseerx.ist.psu.edu)