Recommended medium for the test of Escherichia coli in molecular genetics studiesPackaging Suspend 20 grams of the medium in one liter of distilled water .Mix well and dissolve by heating with frequent agitation. Boil for one minute until complete dissolution. Dispense into appropriate containers and sterilize in autoclave at 121°C for 15 minutes. The prepared medium should be stored at 2-8°C. The color of the prepared medium is clear amber..Formula Tryptone (pancreatic digest of casein) 10 Sodium Chloride 5 Yeast Extract 5