MyTaq™ One-Step RT-PCR Kit incorporates the latest advances in buffer chemistry, including Meridian ultra-pure dNTPs, together with a proprietary reverse transcriptase and MyTaq HS, a new generation of antibody-mediated hot-start DNA polymerase. This ensures that MyTaq One-Step RT-PCR Kit enables fast, highly-specific and ultra-sensitive amplification of RNA targets for use in a broad range of downstream applications. MyTaq One-Step Kit is ideal for determining the presence or absence of RNA templates and quantifying expression through qualitative or semi-quantitative analysis of RNA transcription levels. The one-step format uses gene specific primers to maximize amplification of these targets, whilst eliminating non-specific amplification, ensuring highly efficient and sensitive transcription from as little as 3 pg total RNA. MyTaq One-Step RT-PCR Kit is perfect for the synthesis of double-stranded cDNA products for subsequent gene expression analysis over a broad temperature range, the use of higher temperatures allows reverse transcription through RNA secondary structure, including difficult and GC-rich sequences. Enhanced amplification of these targets ensures high cDNA yields from all RNA, including total RNA, mRNA, in vitro transcribed RNA, snRNA and viral RNA.