For yeast classification based on carbon and nitrogen requirementsPackaging Prepare a 10X solution by dissolving 1.7 grams of the medium in 100 ml distilled water. Mix well. Heat with frequent agitation until complete dissolution. DO NOT BOIL. DO NOT AUTOCLAVE. Sterilize the solution by filtration. Store at 4°C.Prepare the final medium by pipetting, under sterile conditions, 0.5 ml of the 10X solution in 4.5 ml of distilled water. Swirl to mix the solution before inoculation. The color of the prepared medium is amber.Formula Yeast Nitrogen Base 1.7